Frequently Asked Questions
What viral testing is performed on the platelets used to manufacture Clearsate?
In addition to Red Cell Antibody Screening and Bacterial Contamination, we test all platelets used to manufacture Clearsate for: Hepatitis B & C , HIV 1 & 2, Syphilis HTLV I / II, Chagas, West Nile Virus
How is Clearsate filtered?
Clearsate is triple filtered with a 0.2μ final filtration.
Are there any white blood cells in Clearsate?
All platelets used are first leukoreduced to less than 5.0×106/mL.
What is the general range of working concentration of Clearsate to be used when added to cell culture media?
Utility Studies have shown working concentrations to be between 2.0% and 10.0%. Optimal concentrations should be determined by user. Actual concentrations of Clearsate required are often significantly less than FBS.
Does Clearsate come in multiple packaging sizes?
Yes. We currently have 1.0L, 0.5L, 250mL, 125mL, and 50mL product volumes. Please call to discuss other volume or batch requirements.
How is Clearsate to be stored?
Frozen up to 1 year, at ≤ -20˚C.
How does Clearsate performance compare with FBS (Fetal Bovine Serum)?
We recommend that each user of Clearsate first determine the optimal concentrations of Clearsate media supplement for their cells. Utility studies have shown that Clearsate, in optimized concentrations, can perform as good as or better than FBS with many different cell types.
Is Clearsate free of bacterial contamination?
Yes. The lysate is made from platelets that are tested for bacterial contamination. Each batch is sterile filtered and sterility tested.
What growth factors are found in Clearsate?
Clearsate is rich in both Platelet Derived Growth Factor’s (PDGF’s) and (TGF-ß’s).
Are any plant or non-human materials used to make Clearsate?
No. There are absolutely no animal or plant based materials used to make Clearsate.
Does Clearsate incorporate any additives?
No. There are no other additives, or chemicals added which could have any adverse effects on cell growth or expansion. There are also no added antibiotics or preservatives.
Does Clearsate contain anticoagulant or require it to be added for use?
No anticoagulant is used in making Clearsate nor is it required to add any anticoagulant when using Clearsate.
Is Clearsate a plasma or serum based product?
Clearsate is manufactured from saline washed platelets and contains a controlled amount of residual plasma.
What is Clearsate?
Clearsate is a fully human based lysate manufactured from platelets collected from normal healthy blood donors, rich in numerous platelet derived growth factors. It is intended as a replacement for Fetal Bovine Serum and AB Serum.